Figure 1

Plasma SMP30 levels were only elevated in mice with ALF. A: 2-D gel of murine plasma proteins. B: Magnified regions of 2-D gel of murine plasma proteins. The results shown are representative of three independent experiments. The protein spot corresponding to SMP30 is indicated with an arrow. C: Spectrum from LC-ESI-MS/MS analysis of the trypsin digestion products of senescence marker protein 30 (SMP30). D: WB analysis of plasma SMP30 levels in GalN/LPS-treated mice. Lane and gel 1: 5 h after saline injection; lane and gel 2: 5 h after LPS injection; lane and gel 3: 5 h after GalN injection; lane and gel 4: 5 h after GalN/LPS injection; lane and gel 5: 24 h after saline injection; lane and gel 6: 24 h after LPS injection; lane and gel 7: 24 h after GalN injection; lane and gel 8: 24 h after GalN/LPS injection.