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Table 1 Transcription factors and differentiation markers used in this study

From: Multilayered epithelium in a rat model and human Barrett's esophagus: Similar expression patterns of transcription factors and differentiation markers

Antigen Description Expression Pattern Antibody Source Catalogue No. Conc.
p63 Transcription factor essential for squamous epithelium Nuclei of basal and parabasal squamous cells Santa Cruz Biotechnology (Santa Cruz, CA) sc-8431 (mouse mAb) 0.2 μg/ml
Sox2 (SRY-related HMG box gene 2) Transcription factor essential for upper gastrointestinal epithelium Nuclei of epithelial cells in oral cavity, esophagus and stomach Santa Cruz Biotechnology sc-17320 (goat pAb) 4 μg/ml
CK14 (cytokeratin 14) Squamous differentiation marker Cytoplasm of basal squamous cells Novocastra Laboratories Ltd. (Newcastle upon Tyne, UK) NCL-LL002 (mouse mAb) 1:40
CK4 (cytokeratin 4) Squamous differentiation marker Cytoplasm of parabasal squamous cells Sigma-Aldrich (St. Louis, MO) C5176 (mouse mAb) 1:500
Cdx2 (caudal-related homeobox 2) Intestinal transcription factor Nuclei (and occasionally cytoplasm) of columnar and goblet cells BioGenex (San Ramon, CA) MU392-UC (mouse mAb) 1:50
GATA4 (GATA-binding protein 4) Intestinal transcription factor Nuclei of columnar and goblet cells Santa Cruz Biotechnology sc-1237 (goat pAb) 0.25 μg/ml
HNF1α (hepatocyte nuclear factor 1α) Intestinal transcription factor Nuclei of columnar and goblet cells Santa Cruz Biotechnology sc-6547 (goat pAb) 0.4 μg/ml
Villin Intestinal differentiation marker Cytoplasm of columnar and goblet cells NeoMarkers (Fremont, CA) MS-1499 (mouse mAb) 5 μg/ml
Muc2 (mucin 2) Intestinal differentiation marker Cytoplasm of goblet cells Santa Cruz Biotechnology sc-15334 (rabbit pAb) 1:500