Figure 1
Cell proliferation assays for the esophageal adenocarcinoma (TE-7, SEG-1, OE33) or squamous cell cancer (OE21) cell lines after exposure to acid or bile salts. Panel A – [3H]thymidine uptake after exposure to pH 3.5 for the indicated times (3, 5, 10, 15, 20, 60 min, and control) followed by a 24 hour incubation at pH 7.4. Each time point is represented by 8 replicates. Panel B – Cell counts of cells that exclude trypan blue under the same conditions for acid exposure as panel A. Each time point was assayed in duplicate. Shown is one representative experiment out of 3. Panel C – MTT assay for cells treated with acid as described in panel A. Panel D – [3H]thymidine uptake after exposure to either conjugated bile acids or an cocktail of conjugated bile salts (see Methods) for 1 hr followed by incubation in normal media without bile salts for 24 hours. Each time point is represented by 8 replicates. The error bars represent 1 standard deviation.