Immunofluorescence and FACS analysis of RANTES expression. (A) Chang Liver cell lines were stained with anti-human RANTES mAb PE conjugated and processed for FACS analysis. (B) Percentage of cells positive for RANTES expression was plotted in columns. CHWT control indicates vector transfected cells stained with an antibody isotipycally related to anti-RANTES and used as negative control cell line; CHWT indicates same cell line stained with anti-RANTES mAb; CHL are the parental Chang Liver cell line; CH39: core expressing cells; CH352: cells expressing C-E1-E2-NS2-NS3. One representative experiment out of three independently performed is shown; the standard deviation is indicated by the error bars in B plot; statistical significance (p < 0.05) was determined by Student's t-test for differences.