HSP70 expression in the gastric mucosa. (a) Immunostaining of HSP70. HSP70 was induced along the necrotic region and the lower glandular region of the WT mucosa after ethanol injury, whereas it was constitutively expressed in the lower glandular region of the TG mucosa before and after the injury. Ethanol-injured mucosa was stained 6 h after the ethanol administration. Scale: 100 μm. (b) Immunoblotting of HSP70. HSP70 blot was assessed 0, 3, 6, 12 h after the ethanol injury. HSP was induced after ethanol injury in the WT mucosa, while it was constitutively expressed in the TG mucosa before and after the injury.