Ethanol-induced gastric injury in the gastric mucosae. (a) Macroscopic view of the gastric mucosa. Scale: 5 mm. Both mucosae are 6 h after ethanol administration. Note that the WT mucosa displays extensive hemorrhagic lesions, in contrast, the TG mucosa looks undamaged after ethanol administration. (b) Hematoxylin and eosin staining of ethanol-injured WT mucosa (left) and TG mucosa (right). Note the deep ulcer formation in the WT mucosa. Scale: 100 μm. (c) Time course of ethanol-induced gastric injury. Injured area is plotted against time up to 12 h after the ethanol administration. Each point represents an average of five mice. p < 0.05 (d) TUNEL staining of WT mucosa (left) and TG mucosa (right). Both sections are consecutive to those in 2B, respectively. Apoptotic cells are visible with dark-brown nuclei. Numerous apoptotic cells are noted in the WT mucosa. All photos were 6 h after the ethanol administration. Scale: 100 μm.