Figure 5

Immunohistochemistry for Reg3α in wild type and CF mice during acute pancreatitis. Paraffin sections of pancreas from wild type and CF mice, untreated controls or with caerulein-induced pancreatitis, were stained with a Reg3α-specific antibody by the Vectastain ABC technique. Wild type: (A) untreated control; (B) 1 h after a single injection; (C) 7 h after the beginning of seven injections; (D) 3d after the beginning of seven injections. Note reactivity in duct lumina (arrows); and (E) 7d after the beginning of seven injections. CF: (F) untreated control; (G) 1 h; (H) 7 h; (I) 3d; and (J) 7d. Higher magnification images: (K) wild type, 1 h, from the boxed region in panel (B) showing strong expression in a region of inflammation; (L) wild type, 3d, from the boxed region in panel (D) showing expression in a region of fibrosis; (M) untreated CF, from the boxed region in panel (F) showing strong expression in dilated acinar lumina; (N) CF, 1 h, from the boxed region in panel (G) showing expression in acinar cells (arrowhead) and duct lumen (arrow); (O) Omission of the primary antibody (No 1°) as a negative control. This was an adjacent section to that shown in panel (I) focusing on the region of inflammation where specific staining was intense when the primary antibody was included. The scale bar in (J) applies to (A-J). The scale bar in (O) applies to (K-O).