Assessment of RNA quality and levels of 18S RNA in wild type and CF mice during acute pancreatitis. (A) RNA quality. Five micrograms of total RNA were loaded per lane and electrophoresed, the gel was stained with SYBR Green II, and imaged with a Bio-Rad ChemiDoc XRS digital system. Representative of duplicate samples. The time points indicated refer to the elapsed time after the first caerulein injection. (B, C) qRT-PCR quantification of 18S RNA per total RNA. (B) Wild type pancreas samples. (C) CF pancreas samples. The data are means and sem. (*) p < 0.05 compared to control of the same genotype. n = 3 to 7 mice per group and time point.