Severity of acute pancreatitis in wild type and CF mice. (A) Serum amylase. Trunk blood was collected at sacrifice for measurement of serum amylase activity. (B) Tissue water content as a measurement of edema. Pancreatic tissue was obtained at sacrifice and the wet weight determined. The tissue was then lyophilized to dryness and the dry weight was determined. Data are expressed as the water content of the tissue (% wet weight). (C) Pancreatic myeloperoxidase (MPO) activity. Pancreatic tissue was obtained at sacrifice and was then lyophilized to dryness. The tissue was processed and myeloperoxidase (MPO) activity was determined as described in the Materials and Methods. Data are expressed units per g dry weight of the tissue. For (A-C) control mice were untreated or injected with 0.9% NaCl. Mice for the 1 h time point received a single injection of caerulein and were sacrificed one hour after injection. The other data are from mice that received 7 hourly caerulein injections and the times are relative to the first injection. The data are means and sem. (*) p < 0.05 compared to control of the same genotype. (+) p < 0.05 comparing CF to wild type at the same time point. n = 3 to 7 mice per group and time point.