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Figure 1 | BMC Gastroenterology

Figure 1

From: Long-term culture of cholangiocytes from liver fibro-granulomatous lesions

Figure 1

Schistosomal granulomas and outline of method used to isolate bile ductal cell lines. A. Schistosomal egg (arrow) in a portal vein with initial granuloma formation after 40 days of infection (acute phase). Portal space with mieloid progenitors. Hematoxylin-Eosin staining (400×). B. Portal space with a bile duct hyperplasia (arrows) with clear cells after 40 days of infection (acute phase). Hematoxylin-Eosin staining (400×). C. Portal space with biliary ducts (arrows) after 40 days of infection (acute phase). Hematoxylin-Eosin staining (100×). D. Enlarged inflammed portal space containing a hyperplastic bile duct with cristal-like structures (arrow) after 90 days of infection (chronic phase). Note also a Schistosomal egg granuloma (arrowhead). Hematoxylin-Eosin staining (100×). E. Enlarged inflammed portal space containing a hyperplastic bile duct with mucinous metaplasia (arrow) after 90 days of infection (chronic phase). Masson's trichrome staining (400×). F. Hyperplastic bile ducts with mucinous metaplasia (arrow) surrounded by eosinophyls and myeloid progenitors after 90 days of infection (chronic phase). Hematoxylin-Eosin staining (400×).G. Diagram outlining the different sources used to isolate bile ductal cell lines.

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