Figure 3
Inhibition of H,K-ATPase activity by arachidonic acid (A) and prostaglandin E 2 (B). Gastric microsomes suspended in ATPase assay buffer at pH 7.4 were incubated for 30 min with varying concentrations of arachidonic acid or PGE2 and ATPase activity was then measured at pH 7.4. IC50 for arachidonic acid was 16.7 μM (CI = 8.9 – 31.5 μM), and the IC50 for PGE2 was 15 μM (CI = 3.96 – 57.3 μM). The data-points show mean ± s.e. from three independent assays in each of which SCH28080-sensitive ATPase activity was measured in triplicate.