Immunolocalization of ABCG5 and ABCG8 in human intestinal sections. Human intestinal surgical samples were stained with ABCG5 (panel A, red staining) or ABCG8 (panel B, green staining). The layout is as indicated for figure 3. At the cellular level, ABCG5 was expressed in a more diffuse pattern (panel A, see bottom right hand panel). In contrast, ABCG8 was expressed in the apical surfaces of the enterocytes (panel B, bottom right hand panel). Both proteins seemed to be expressed only in the enterocytes lining the villi and no significant expression was detected in any of the other cell layers.