Immunolocalization of ABCG5 and ABCG8 in human gall bladder sections. Gall bladder surgical samples were stained for ABCG5 (panel A, red staining) or ABCG8 (panel B, green staining). The layout is as indicated for figure 3. Both ABCG5 and ABCG8 appeared to be confined to the epithelial cells lining the lumen and no significant staining in any of the deeper cell layers was detected. At the cellular level, both proteins seem to be diffusely expressed within the cell and at plasma membrane. A strict apical expression was not observed for either of these proteins.