Co-localization of ABCG5 and ABCG8 with MRP2 by immunohistochemistry. Liver sections were simultaneously incubated with either the combination of anti-ABCG5/MRP2 or anti-ABCG8/MRP2. Panel A shows staining patterns of MRP2 and ABCG5 independently (upper portion) then merged together (lower right) with what appears to be similar over-lapping expression patterns. Likewise MRP2 and ABCG8 expression patterns appear to over lap as well as shown in panel B. Bar is 20 μm.