Jejunal microvilli atrophy and reduced nutrient transport in rats with advanced liver cirrhosis: improvement by Insulin-like Growth Factor I

Table 2 Kinetic constants (Maximal velocity – V_max- and the affinity transporter constant – K_t-) for galactose and amino acid uptake by BBMVs in the three experimental groups:

Nutrient	Kinetic constants	Control group (CO, n = 10)	Untreated cirrhotic rats (CI, n = 10)	Cirrhotic rats treated with IGF-I (CI+IGF, n = 10)
D-galactose	Vmax	3470 ± 30	2460 ± 24***	3008 ± 45*
	Kt	0.71 ± 0.01	1.03 ± 0.02**	0.78 ± 0.02
L-leucine	Vmax	305 ± 19	210 ± 15**	283 ± 18
	Kt	1.6 ± 0.1	2.0 ± 0.2**	1.1 ± 0.1
L-proline	Vmax	165 ± 32	92 ± 7	159 ± 28
	Kt	1.9 ± 0.2	3.3 ± 0.2***	2.1 ± 0.1
L-glutamic acid	Vmax	828 ± 23	545 ± 13***	789 ± 18
	Kt	0.6 ± 0.0	1.1 ± 0.0**	0.8 ± 0.0
L-cysteine	Vmax	195 ± 26	105 ± 11***	182 ± 19
	Kt	1.9 ± 0.1	2.3 ± 0.2**	1.9 ± 0.1

Values are mean ± SEM, n = pool of BBMVs from 10 animals from each group. V_max expressed as pmol of substrate/ mg protein × 3 seconds and K_t as mmol/l. *p < 0.05, **p < 0.01, ***p < 0.001 CO vs other groups.

ISSN: 1471-230X