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Figure 1 | BMC Gastroenterology

Figure 1

From: A novel mutation of the calcium sensing receptor gene is associated with chronic pancreatitis in a family with heterozygous SPINK1 mutations

Figure 1

Melting curve analysis ( SPINK1 N34S) of the index patient and family members To assess the SPINK1 genotype, representative gene fragments of the SPINK1 gene were amplified by PCR. The melting point (Tm) of each amplified double-stranded DNA fragment was determined using the LightCycler thermocycling device and designated probes. Curves with a melting point at 58,53°C represent the SPINK1 (N34S) control, index patient (II:4), daughter of the index patient (III:1), healthy brother (II:3) and healthy mother of the index patient (I:2). The melting point curve with the higher melting point at 63,17°C, due to a more stable complex (donor probe was complimentary to the wild type) represents the SPINK1 wild type.

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