Secretion of human IL-10 concentration by transfected endothelial cells. There was no detectable human IL-10 signal in the non-transfected cell medium (control). However, the medium from the IL-10 transfected SVEC medium showed a large and significant increase in the IL-10 concentration (1209 ± 2 pg/ml) at 48 h after IL-10 gene transfection (n = 5). (# p < 0.05 from control). One-way ANOVA with Fisher's PLSD test.