MAPK protein and phosphorylated MAPK were markedly elevated in extract of HCCs as compared with ABL tissues. Human HCCs (T) and ABL tissues (N) were collected and tissue lysate was prepared as described under Experimental Procedures. Samples (100 μg protein/sample) were subjected to Western blot analysis as described . Blots were incubated with mouse anti-MAPK and mouse anti-phospho-MAP kinase (Thr202/Tyr204) (A, B and C) (A, B and C) and mouse anti-α-tubulin antibodies. The ratio of phosphorylated MAPK to MAPK is shown in (D). Bars with different letters are significantly different from one another at p < 0.01 as determined by ANOVA test. All the samples in A &B are paired and samples in C are individual.