Figure 6

Effects of LPS from different bacteria on TNF-α secretion Release of TNF-α into the apical and basolateral medium by cultured MGBE exposed to 100 μg/mL LPS from various bacteria for two hours. TNF-α was measured by immunoassay and calculated as the total pg present in the apical and basolateral medium. Each treatment was done in triplicate and assay repeated twice more with E. coli LPS and once more with P. aeruginosa and K. pneumoniae LPS. Values represent the mean of each treatment (n = 9 for E. coli LPS and n = 6 for P. aeruginosa and K. pneumoniae LPS) ± standard error. There was a statistical difference between the amount of TNF-α in the basal versus apical medium as measured by paired Student's t-test (* = p < 0.01).