The effects of glucose on the nuclear morphology of LPS-treated DC2.4 cells in vitro . (A) Hoechst 33258 staining of DC2.4 cells treated with LPS for 24 h (400×). Cells treated with a high concentration of glucose showed obvious apoptotic features including cytoplasm concentration, chromatin condensation and multiple chromatin fragments compared with cells without glucose. Brightly stained nuclei represent cells undergoing apoptosis. (B) Localization of AKT, ERK, Bax and Bcl-2 in DCs (400×). CTR is negative control treated with the second antibody alone. No immunoreactive cells were detected in the CTR. Actin staining was used as positive control. High glucose elevated Bax expression but decreased the expression of Bcl-2 and the phosphorylation of AKT and ERK.