Association of CN2 expression with colon cancer cell cycle progression and tumorigenicity. (A) Cell-cycle phase distributions were analyzed by a FACScalibur flow cytometer. These experiments were repeated 3 times, and the symbols represent the mean values of triplicate tests (mean ± SD). (B) Western blotting analysis of cyclin E, cyclin B1 and EGFR expression. The experiment was independently repeated at least 3 times. (C) Knockdown of CNDP2 inhibited tumor formation in nude mice. Nude mice were inoculated with RKO stably transfected with empty vector and shRNA_CNDP2 expression vectors. *p < 0.05.