Figure 1

Hematoxylin-Eosin (H&E, 1st and 2nd rows), Masson’s trichrome (3rd and 4th rows) and Sirius red (5th and 6th rows) – stained sections of liver tissues from chow diet, chow diet + 0.5 ml/Kg B.wt CCl _4, fast food diet (FFD), FFD + 0.5 ml/Kg B.wt CCl ₄ fed animals on 8th week. There was no steatosis, hepatocellular ballooning or fibrosis in animals fed with chow diet. Mild micro-vesicular fatty changes and mild hepatocellular ballooning, without fibrosis observed in animals fed with chow diet + 0.5 ml/Kg B.wt CCl₄. Fast food fed animals without CCl₄ showing moderate micro-vesicular and macro-vesicular fatty changes, hepatocellular ballooning and minimal perisinusoidal fibrosis. FFD + 0.5 ml/Kg B.wt CCl₄ fed animals showing severe micro-vesicular and macro-vesicular fatty changes with significant hepatocellular ballooning and prominent perisinusoidal, pericellular region with extensive distribution and bridging fibrosis. All liver sections were evaluated at X10 (low-magnification) and X40 (high-magnification).