Silencing SLC38A1 with siRNA in SH-10-TC cells and experiments in vitro. (A) Representative Western blot in the siRNA experiments. siRNA #2 reduced the SLC38A1 transcript by >70% in comparison to scrambled control (lane1 and 6). GAPDH was used as the internal control. (B) CCK-8 assay shows si-SLC38A1 significantly inhibited the cell growth of SH-10-TC cells. (C) Upper panel: si-SLC38A1 decreased the migration of SH-10-TC cells in transwell chambers, compared to parental cells and si-Ctl group. Lower panel: migrated cells on the surface of the membrane (Columns, mean; bars, SD. *, P < 0.01 vs. scrambled control).