Quantification of in vitro transduction of vesicular stomatitis virus G protein (VSV-G)-pseudotyped lentivirus (LV) encoding mouse interleukin 10 (mIL10) in colonic cell lines. Cell line production of mIL-10 in pg/ml following three titered infectious doses of LV (0.1 ng/p24, 1.0 ng/p24, 10 ng/p24) for transfection are shown. Each experiment collected took triplicate supernatant, and measured IL-10 by ELISA. All values were expressed as mean ± SD. *p < 0.05 compared with the results of 0.1 ng p24 LV transduction.