Effect of treatment with anti-HMGB1 antibody or sham IgG on hepatic proliferation in mice challenged with acetaminophen overdose. PCNA staining was assessed 24 and 48 hours after acetaminophen administration. Animal model and treatment were the same as described in Figure 1 (n = 6 for each group at 24 h time point, n = 9-10 for each group at 48 h. A = sham IgG at 24 h, B = anti-HMGB1 at 24 h, C = sham IgG at 48 h, D = anti-HMGB1 at 48 h, arrows indicate those PCNA-positive nuclei at high power 400×). Figure 6
E and F: Effect of treatment with sham or anti-HMGB1 antibodies on hepatocyte regeneration in acetaminophen injected mice. Animal model and treatment were the same as the 48 h experiment described in Figure 1 except that mice from the 48 h groups were administered 5-bromo-2-deoxyuridine (BrdU, 50-mg/kg i.p. injection at 46 h time point). BrdU staining was assessed at 48 h (n = 6 for each group, E = sham IgG at 48 h, F = anti-HMGB1 at 48 h, magnification = 200× for A, B, C, D, E and F). A typical picture is shown.