Effect of treatment with anti-HMGB1 or sham IgG on the mRNA expression of hepatic TNF-α and IL-6 in mice challenged with acetaminophen overdose. 24 and 48 hours after APAP administration, relative messenger RNA levels of (A) TNF-α and (B) IL-6 were detected using real-time PCR. Animal model and treatment were the same as described in Figure 1. Baseline (control) levels of cytokines were measured 24 and 48 hrs after saline injection. *indicates p < 0.05 (analysis of variance followed by Fisher’s LSD test). GAPDH = glyceraldehydes-3-phosp dehydrogenase.