Loss of alleviation of intestinal permeability of LPKM induced by EPEC ex vivo . (A) The small intestinal permeability to mannitol in the IL-10-/- mice was increased, compared with the wild-type group. In LP group, the small intestinal permeability to mannitol significantly decreased, compared with control (P < 0.05). However, in LPKM group, the small intestinal permeability to mannitol did not changed significantly (P > 0.05). (B) Similar results were indicated for intestinal resistance. (C & D) Similar results were observed in colonic tissues. Colonic permeability to mannitol increased in the IL-10-/- mice with a corresponding decrease in TER, both of which were prevented by LP (P < 0.05). However, in LPKM group, colonic permeability to mannitol and TER did not changed significantly, compared with control (P > 0.05). Each group used 10 animals for determination. *vs. EPEC group, P < 0.05; # vs. *, P < 0.05. The data were expressed as the mean ± standard deviation. Statistical analyses were performed using the SPSS 13.0 software (SPSS Inc., Chicago, IL). Data were analyzed by one-way ANOVA when conditions of homogeneity of variance were present.