Validation of the knockout of MIMP protein in LPKM and the adhesion of EPEC or LP to NCM460 cells. (A) PCR amplicons over the integrated membrane protein region in LPKM confirmed the loss of ~ 200 bp in the genes surrounding the deletion; (B) Western blotting confirmed the non-expression of MIMP protein section in the whole bacteria protein of LPKM; (C) Adhesion of LP to NCM460 cells. The adhesion rate of LP to NCM460 cells was lower compared with the LPKM; (D) Adhesion of EPEC to NCM460/MIMP cells. The adhesion rate of EPEC to NCM460 cells was reduced when LP was added, while addition of LPKM had no effect about the inhibition of EPEC adhesion. However, the anti-MIMP deprived the effect of LP on the reduction of EPEC adhesion. *, P < 0.05, vs. Control; #, P < 0.05, vs. *group(s).