Figure 2

Representative ventral images of fluorescence emission of gastric contents. Mice received a bolus of 10 μL/g body weight of liquid EN (Hine® E-gel) containing 1.25 pmol of GastroSense™750 fluorescence imaging agent without (A) or with (B) pectin. Representative ventral images of anesthetized mice are shown at baseline and at 5, 15, 30, 45, and 60 min later to monitor gastric EN. All images were acquired using the same pseudocolor scale of radiance to show relative changes in bioluminescence emission over time.