Figure 6

Analysis of mitochondrion in primary hepatocytes. (A) The morphologic features of the mitochondrion were observed by transmission electron microscopy. The changes in mitochondria were dependent on the concentration of ammonia exposure. We observed that the mitochondria showed irregular swelling, with fractured cristae and vacuolar degeneration. (B) C1: The position of peak shift in the control group at 0 min. C2: The position of peak shift in the control group at 15 min. C3: The position of peak shift in the control group at 30 min. T1: The peak shift position of the primary hepatocyte group with 20 mmol/L NH₄Cl at 0 min. T2: The peak shift position of the primary hepatocyte group with 20 mmol/L NH₄Cl at 15 min. T3: The peak shift position of the primary hepatocyte group with 20 mmol/L NH₄Cl at 30 min. The position of peak shift in the same group gradually moved to left with the passage of time, while at the same period of time, the position of peak shift in the hyperammonia group moved to left more significantly than that in the control group.