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Figure 2 | BMC Gastroenterology

Figure 2

From: High dose multiple micronutrient supplementation improves villous morphology in environmental enteropathy without HIV enteropathy: results from a double-blind randomised placebo controlled trial in Zambian adults

Figure 2

Example of morphometric analysis. Morphometry on a jejunal biopsy demonstrating relatively mild features of EE, with villous blunting and lymphocyte infiltration (arrows). H&E stained sections at a magnification of x8 were digitised. Automatic threshold definition was used to measure villous area (VA; area within blue lines) and villous perimeter (VP; length of blue lines excluding base). Villous height (VH; green), crypt depth (CD; cyan), maximal villous width perpendicular to VH (VW; yellow), and muscularis mucosal length (white; used as a denominator for VP and VA measurements) were drawn by eye using the cursor on the computer monitor. See also [2].

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