Figure 3
Acetaminophen (APAP)-induced centrilobular necrosis in BALB/c mice is attenuated by the application of a cholinesterase inhibitor. Liver injury was induced with 600 mg/kg APAP i.p.. After 1 and 7 hours 80 μg/kg neostigmine or vehicle control was applied. Liver tissue was harvested 12 hours after application of APAP and processed for histopathology. Representative H & E-stained liver sections (100× magnification), (A) control mice, (B) APAP + solvent, (C) APAP + neostigmine. (D) Area of necrosis (% of area). (E) Hemorrhage [0–3] (mean ± SD; open bars, control mice, hatched bars, APAP + solvent, filled bars, APAP + neostigmine; n = 6 for each group; *p < 0.05, **p < 0.01 APAP-alone-treated mice vs. APAP + neostigmine-treated mice).