Hydrogen-enriched saline treatment protects liver against I/R injury. Male Sprague Dawley rats were subject to partial warm liver ischemia/reperfusion injury with intraperitoneal injection of either normal saline or hydrogen enriched saline at dose of 2.5, 5 or 10 ml/kg 10 minutes before reperfusion. Liver damage was assessed 2 hours after reperfusion with paraffin sections stained with H&E (original magnification × 200) and serum ALT level measurement. An example of microphotograph from (A) a sham-operated animal, (B) an animal undergoing 60 minutes of ischemia followed by 2 hours of reperfusion without any treatment, (C) with normal saline (10 ml/kg), or (D) hydrogen-enriched saline (10 ml/kg). (E) The dose-response (2.5-10 ml/kg) of hydrogen-enriched saline treatment and 10 ml/kg saline treatment on serum ALT release. Sham-operated animals underwent laparotomy only. Mean ± SEM (n = 8), *p < 0.05. (F) The time-course of liver damage assessed with ALT measurement at 2, 6, 12 and 24 hours after reperfusion with normal saline or hydrogen enriched saline treatment (10 ml/kg).