Chronic stress induces hepatic TG and TC accumulation. After mice were anesthetized, whole liver were removed and weighed. Hepatic lipids were extracted, and TG, TC concentrations were measured. (A) Hepatic index = Liver weight (mg)/body weight (g). (B) The levels of serum TC and TG content are represented in bar chart, respectively. (C) Liver TG and TC concentrations in stress group and control group. (D) Representative slides show hematoxylin and eosin (H&E)-stained and Oil red O-stained liver sections from control mice or stress mice for 12 weeks. The percentage of the area occupied by oil red O–stained lipid droplets was calculated using Image J, averaging 3–5 separate, randomly selected 40× fields. HE sections show chronic stress causing hepatocytes ballooning (yellow arrows). In addition, there are foci of inflammatory cell infiltration and lipid deposits (white arrows) Original magnification, ×100. Scale bars: 50 μm. Error bars represent standard error (SE). *P < 0.05, ***P < 0.001 versus control mice.