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Table 1 Primers and probes and annealing temperatures for detection of GST polymorphisms

From: No role for glutathione S-transferase genotypes in Caucasian esophageal squamous cell or adenocarcinoma etiology: an European case–control study

GST

Polymorphism protein change

Genotypes

Primers (forward) probes (wild type)

Primer (reverse) probes mutant)

Annealing temp. MgCl 2 Conc.

GSTM1

Deletion

Wild Type: GSTM1 +

5’-CTC CTG ATT ATG ACA GAA GCC-3’

5’-CTG GAT TGT AGC AGA TCA TGC-3’

58°C

Polymorphism

Hetero.: GSTM1 +

  

2.0 mM

 

Homoz.: GSTM1 -

   

GSTA1

-631 T > G

Wild Type: GSTA1 *a/*a

5’-TGT TGA TTG TTT GCC TGA AAT T-3’

5’-GTT AAA CGC TGT CAC CGT CC-3’

58.5°C

-567 T > G

Hetero.: GSTA1 *a/*b

  

2.0 mM

-69 C > T, -52 G > A

Homoz.: GSTA1 *b/*b

   

GSTP1

313 A > G

Wild Type: I-105-I

5’-CCT GGT GGA CAT GGT GAA TGA-3’

5’-AGC CAC CTG AGG GGT AAG-3’

64°C

105 Ile > Val

Hetero.: I-105-V

5 ’- Fam - CGC TGC AAA TAC ATC TCC CTC ATC TAC A - BHQ1 - 3

5 ’- Hex - CGC TGC AAA TAC GTC TCC CTC ATC TAC A - BHQ1 - 3

2.5 mM

 

Homoz.: V-105-V

   

GSTP1

341 C > T

Wild Type: A-114-A

5’- TGGACAGGCAGAATGGAATAGAG -3’

5’- GGGTCTCAAAAGGCTTCAGTTG -3’

65°C

114 Ala > Val

Hetero.: A-114-V

5 ’- Fam - CATCCTTGCCCGCCTCCTGCCAGA - BHQ1 - 3

5 ’- Hex - CATCCTTGCCCACCTCCTGCCAGA - BHQ1 - 3

3.0 mM

 

Homoz.: V-114-V

   

GSTT1

Deletion

Wild Type: GSTT1 +

5’-TTC CTT ACT GGT CCT CAC ATC TC-3’

5’-TCA CCG GAT CAT GGC CAG CA-3’

59.5°C

Polymorphism

Hetero.: GSTT1 +

  

2.0 mM

 

Homoz.: GSTT1 -