|
GSTM1
|
Deletion
|
Wild Type: GSTM1 +
|
5’-CTC CTG ATT ATG ACA GAA GCC-3’
|
5’-CTG GAT TGT AGC AGA TCA TGC-3’
|
58°C
|
|
Polymorphism
|
Hetero.: GSTM1 +
| | |
2.0 mM
|
| |
Homoz.: GSTM1 -
| | | |
|
GSTA1
|
-631 T > G
|
Wild Type: GSTA1 *a/*a
|
5’-TGT TGA TTG TTT GCC TGA AAT T-3’
|
5’-GTT AAA CGC TGT CAC CGT CC-3’
|
58.5°C
|
|
-567 T > G
|
Hetero.: GSTA1 *a/*b
| | |
2.0 mM
|
|
-69 C > T, -52 G > A
|
Homoz.: GSTA1 *b/*b
| | | |
|
GSTP1
|
313 A > G
|
Wild Type: I-105-I
|
5’-CCT GGT GGA CAT GGT GAA TGA-3’
|
5’-AGC CAC CTG AGG GGT AAG-3’
|
64°C
|
|
105 Ile > Val
|
Hetero.: I-105-V
|
5
’-
Fam
-
CGC TGC AAA TAC ATC TCC CTC ATC TAC A
-
BHQ1
-
3
’
|
5
’-
Hex
-
CGC TGC AAA TAC GTC TCC CTC ATC TAC A
-
BHQ1
-
3
’
|
2.5 mM
|
| |
Homoz.: V-105-V
| | | |
|
GSTP1
|
341 C > T
|
Wild Type: A-114-A
|
5’- TGGACAGGCAGAATGGAATAGAG -3’
|
5’- GGGTCTCAAAAGGCTTCAGTTG -3’
|
65°C
|
|
114 Ala > Val
|
Hetero.: A-114-V
|
5
’-
Fam
-
CATCCTTGCCCGCCTCCTGCCAGA
-
BHQ1
-
3
’
|
5
’-
Hex
-
CATCCTTGCCCACCTCCTGCCAGA
-
BHQ1
-
3
’
|
3.0 mM
|
| |
Homoz.: V-114-V
| | | |
|
GSTT1
|
Deletion
|
Wild Type: GSTT1 +
|
5’-TTC CTT ACT GGT CCT CAC ATC TC-3’
|
5’-TCA CCG GAT CAT GGC CAG CA-3’
|
59.5°C
|
|
Polymorphism
|
Hetero.: GSTT1 +
| | |
2.0 mM
|
| |
Homoz.: GSTT1 -
| | | |
