No role for glutathione S-transferase genotypes in Caucasian esophageal squamous cell or adenocarcinoma etiology: an European case–control study

Table 1 Primers and probes and annealing temperatures for detection of GST polymorphisms

GST	Polymorphism protein change	Genotypes	Primers (forward) probes (wild type)	Primer (reverse) probes mutant)	Annealing temp. MgCl ₂ *Conc.*
GSTM1	Deletion	Wild Type: GSTM1 +	5’-CTC CTG ATT ATG ACA GAA GCC-3’	5’-CTG GAT TGT AGC AGA TCA TGC-3’	58°C
	Polymorphism	Hetero.: GSTM1 +			2.0 mM
		Homoz.: GSTM1 -
GSTA1	-631 T > G	Wild Type: GSTA1 a/a	5’-TGT TGA TTG TTT GCC TGA AAT T-3’	5’-GTT AAA CGC TGT CAC CGT CC-3’	58.5°C
	-567 T > G	Hetero.: GSTA1 a/b			2.0 mM
	-69 C > T, -52 G > A	Homoz.: GSTA1 b/b
GSTP1	313 A > G	Wild Type: I-105-I	5’-CCT GGT GGA CAT GGT GAA TGA-3’	5’-AGC CAC CTG AGG GGT AAG-3’	64°C
	105 Ile > Val	Hetero.: I-105-V	5 ’- *Fam* - *CGC TGC AAA TAC ATC TCC CTC ATC TAC A* - *BHQ1* - 3 ’	5 ’- *Hex* - *CGC TGC AAA TAC GTC TCC CTC ATC TAC A* - *BHQ1* - 3 ’	2.5 mM
		Homoz.: V-105-V
GSTP1	341 C > T	Wild Type: A-114-A	5’- TGGACAGGCAGAATGGAATAGAG -3’	5’- GGGTCTCAAAAGGCTTCAGTTG -3’	65°C
	114 Ala > Val	Hetero.: A-114-V	5 ’- *Fam* - *CATCCTTGCCCGCCTCCTGCCAGA* - *BHQ1* - 3 ’	5 ’- *Hex* - *CATCCTTGCCCACCTCCTGCCAGA* - *BHQ1* - 3 ’	3.0 mM
		Homoz.: V-114-V
GSTT1	Deletion	Wild Type: GSTT1 +	5’-TTC CTT ACT GGT CCT CAC ATC TC-3’	5’-TCA CCG GAT CAT GGC CAG CA-3’	59.5°C
	Polymorphism	Hetero.: GSTT1 +			2.0 mM
		Homoz.: GSTT1 -