GST | Polymorphism protein change | Genotypes | Primers (forward) probes (wild type) | Primer (reverse) probes mutant) | Annealing temp. MgCl 2 Conc. |
---|---|---|---|---|---|
GSTM1 | Deletion | Wild Type: GSTM1 + | 5’-CTC CTG ATT ATG ACA GAA GCC-3’ | 5’-CTG GAT TGT AGC AGA TCA TGC-3’ | 58°C |
Polymorphism | Hetero.: GSTM1 + | 2.0 mM | |||
Homoz.: GSTM1 - | |||||
GSTA1 | -631 T > G | Wild Type: GSTA1 *a/*a | 5’-TGT TGA TTG TTT GCC TGA AAT T-3’ | 5’-GTT AAA CGC TGT CAC CGT CC-3’ | 58.5°C |
-567 T > G | Hetero.: GSTA1 *a/*b | 2.0 mM | |||
-69 C > T, -52 G > A | Homoz.: GSTA1 *b/*b | ||||
GSTP1 | 313 A > G | Wild Type: I-105-I | 5’-CCT GGT GGA CAT GGT GAA TGA-3’ | 5’-AGC CAC CTG AGG GGT AAG-3’ | 64°C |
105 Ile > Val | Hetero.: I-105-V | 5 ’- Fam - CGC TGC AAA TAC ATC TCC CTC ATC TAC A - BHQ1 - 3 ’ | 5 ’- Hex - CGC TGC AAA TAC GTC TCC CTC ATC TAC A - BHQ1 - 3 ’ | 2.5 mM | |
Homoz.: V-105-V | |||||
GSTP1 | 341 C > T | Wild Type: A-114-A | 5’- TGGACAGGCAGAATGGAATAGAG -3’ | 5’- GGGTCTCAAAAGGCTTCAGTTG -3’ | 65°C |
114 Ala > Val | Hetero.: A-114-V | 5 ’- Fam - CATCCTTGCCCGCCTCCTGCCAGA - BHQ1 - 3 ’ | 5 ’- Hex - CATCCTTGCCCACCTCCTGCCAGA - BHQ1 - 3 ’ | 3.0 mM | |
Homoz.: V-114-V | |||||
GSTT1 | Deletion | Wild Type: GSTT1 + | 5’-TTC CTT ACT GGT CCT CAC ATC TC-3’ | 5’-TCA CCG GAT CAT GGC CAG CA-3’ | 59.5°C |
Polymorphism | Hetero.: GSTT1 + | 2.0 mM | |||
Homoz.: GSTT1 - |