Figure 4
From: Deficiency in four and one half LIM domain protein 2 (FHL2) aggravates liver fibrosis in mice
Transdifferentiation of isolated HSC to MFB. HSC from wt and FHL2 deficient mice were isolated by FACS-based protocols and plated on plastic surfaces for 2 or 7 days. For generation of fully transdifferentiated MFB, the cells were passaged once and cultured for an additional 2 or 7 day period. At indicated time points, the cells were fixed in 4% paraformaldehyde and stained with a rhodamine-phalloidin-conjugate. Note the typical appearance of F-actin fibres in both cell phenotypes that are highly characteristic for activated HSC and transdifferentiated MFB.