Figure 2

Effect of remifentanil pretreatment on the jejunum and the ileum after ischemia-reperfusion or sham laparotomy. Photomicrographs depicting H&E-stained intestinal sections of mice pretreated with saline (C jejunum, D ileum) or remifentanil (E jejunum, F ileum) after ligation of the superior mesenteric artery for 30 min and reperfusion of 60 min. Representative photomicrographs of remifentanil or saline pretreated sham-operated controls are also displayed (A jejunum, B ileum). n = 9 for saline and n = 7 for remifentanil (IR group); n = 4 for each treatment (Sham group). Red arrows point to severe injury with denuding of villi tips (grade 3). Black arrows indicate Gruenhagen’s spaces, a sign of mild ischemia-reperfusion injury (grade 1). Magnification 100 ×; scale bars indicate 100 μm.