Figure 1
From: Activation of Liver FGF21 in hepatocarcinogenesis and during hepatic stress
Liver is the source of FGF21 elevation upon dietary restriction and liver damage. The FGF21 expression level was analyzed by quantitative PCR in the indicated mouse tissues under different conditions. A : Increase of FGF21 mRNA levels specifically in mouse livers after prolonged starvation for 48 hrs. mRNA levels are expressed as fold changes relative to the internal β-actin levels in the respective tissues. WAT: white adipose tissue. BAT: brown adipose tissue. Data are shown as mean ± sd, *p (n = 6) <0.05. B : Induction of hepatic FGF21 mRNA in the liver after PHx. mRNA levels at different time lapses after PHx are expressed as fold changes relative to that before PHx. *p <0.05 (n = 3 for each data point). Inset: the corresponding serum FGF21 protein levels were determined by soluble KLB (sKLB) pulldown and western blotting analyses as described in the Method section. C : Changes of hepatic KLB mRNA levels after PHx. D : Changes of hepatic FGFR4 mRNA levels after PHx. Total RNA samples for analyses of KLB and FGFR4 expression are the same as for FGF21 in (B).