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Figure 2 | BMC Gastroenterology

Figure 2

From: Comparative proteomics study on liver mitochondria of primary biliary cirrhosis mouse model

Figure 2

Procedure of quantitative iTRAQ proteomics. (A) Strategy for comparative proteomics study on the liver mitochondria between mouse models and controls. (B) Evaluation of mitochondrial purity with organelle specific antibodies. (C) An example of the spectra, showing peptide (ALALAQEILPQAPIAVR) which is one of the three unipeptides for Isoform 1 of Enoyl-CoA hydratase domain-containing protein 2 (ECHDC2). (D) Distribution of peptides in each fraction according to their isoelectric points. Error bars show 95% confidence interval. (E) Quantities of peptide spectra and proteins in each fraction.

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