Figure 9

FACS analysis. Panel 1A (FACS analysis of CD3⁺CD4⁺ and CD3⁺CD8⁺ cells of liver samples): Representative liver samples from C57Bl/6 wild-type (i and ii) and IFN-γ^−/− on C57Bl/6 background (iii and iv) mice 12 hr after injection with 12 mg/kg of ConA. The number of mononuclear cells per mouse isolated from ConA injected mice was 1.5 × 10⁶ (C57Bl/6) and 1.8 × 10⁶ (C57Bl/6 IFN-γ^−/−), respectively. The number of mononuclear cells per mouse isolated from uninjected C57Bl/6 mice was 5.0 × 10⁴ (v and vi). The values in the upper right corners (i-vi) are percents of CD4⁺ or CD8⁺ T cells. Panel 1B: The numbers (×10⁶) of CD4⁺ (lines) and CD8⁺ (dots) T cells for wild-type (WT) and IFN-γ^−/− mice are shown. There were no significant differences between the numbers of CD4⁺ or CD8⁺ T cells isolated from the two strains. As expected, there was near absence of these cells in the uninjected mice. Panel 2A-B (FACS analysis of CD3 ⁺ NKT ⁺ and CD45 ⁺ CD11b ⁺ cells of liver samples). Note the significant number of NKT⁺ cells (lines) present in the WT mice compared to the IFN-γ^−/− mice (p<0.005). There was no significant difference between the numbers of CD11b⁺ cells (dots) isolated from the two strains. As expected, there was near absence of these cells in the uninjected mice.