Table 2 Primers used for real-time qPCR
Target group | Primer | Sequence (5′-3′) | Annealing temperature (°C) | Reference |
|---|---|---|---|---|
Enterobacteriaceae | Eco1457F | CATTGACGTTACCCGCAGAAGAAGC | 63 | [18] |
Eco1652R | CTCTACGAGACTCAAGCTTGC | |||
Veillonella | Vei343F | A(C/T)CAACCTGCCCTTCAGA | 62 | [18] |
Vei343F | CGTCCCGATTAACAGAGCTT | |||
Bacteroides | Bac566F | GGGTTTAAAGGGAGCGTAGG | 53 | [19] |
Bac692R | CTACACCACGAATTCCGCCT | |||
Clostridium | CI-F | TACCHRAGGAGGAAGCCAC | 54 | [20] |
CI-R | GTTCTTCCTAATCTCTACGCAT |