Function and metabolism analysis of fecal microbiota from HBLC patients and control samples. Blue bars represent the control samples, and red bars represent the HBLC samples. The Student t test was used to evaluate the statistical difference between the two groups. *P < 0.05; ** P < 0.01 (A) Genes annotated using the eggNOG database. The significantly differential genes were annotated as: E, amino acid transport and metabolism; P, inorganic ion transport and metabolism; Q, secondary metabolites biosynthesis, transport and catabolism; and W, extracellular structures. (B) Genes annotated using the KEGG database. The significantly differential genes were annotated with pathways: Cellular, cell growth and death; Environmental, membrane transport; Environmental, signal transduction; Metabolism, biosynthesis of other secondary metabolites; Metabolism, energy metabolism; Metabolism, enzyme families; Metabolism, metabolism of other amino acids; and Metabolism, xenobiotics biodegradation and metabolism. (C) Relative abundance of genes annotated as BSH related to primary and secondary bile acid biosynthesis [KEGG: K01442]. Green bars represent the control samples, and red bars represent the HBLC samples. The Student t test was used to evaluate statistical difference between the two groups, P = 0.013.