Saturated fatty acids increase fatty acid accumulation in the liver. Triglycerides (A) were measured with a colorimetric kit in the liver. Liver weight (B), final body weight (C) and liver to body ratio (D) are shown. Oil-Red-O staining shows representative fatty acid accumulation in the liver (E). Haematoxylin eosin staining shows liver histology (F). a
P < 0.05 ANOVA result for the effect of the MUFA diet compared to SFA; bPUFA compared to SFA. Means ± SEM, n = 5. Abbreviations: C, control; SFA, saturated fatty acids; MUFA, monounsaturated fatty acids; PUFA, polyunsaturated fatty acids.