Effects of IL-4 or IL-10 transfection on treatment of TNBS-induced murine colitis. IL-4 or IL-10 treatment attenuated colonic inflammation and reduced histological colitis scores in TNBS-induced murine colitis. In contrast, their combination has minimal effect on preventing colitis development. A: Hematoxylin and eosin staining of the colon. Group 1, treated with TNBS only; Group 2, treated with pcDNA3.0; Group 3, treated with IL-4; Group 4, treated with IL-10; Group 5, treated with combination. Magnification, ×200. Scale bar = 100 μm. B: Histologic scores of the colon tissues. Each tissue section was evaluated for an inflammatory score from 0 to 4 and the data are expressed as median (*p < 0.05 compared to the TNBS-only group using Kruskal Wallis test).