Quantitative RT-PCR detection of IL-4 and IL-10 expression in vivo . Colon tissue samples were removed from TNBS-treated murine colitis on day 7 and subjected to RNA isolation and qRT-PCR. A: The expression of IL-4 mRNA in vivo. B: The level of IL-10 mRNA in vivo. The transgenic expression of IL-4 or IL-10 was successfully induced. All data are expressed as mean ± s.d.