Figure 7

Effects observed in ILK-ko mice are preserved at day 14 post-infection. ILK-ko and control mice were gavaged with C. rodentium and their cecal and colonic tissues assessed (day 14) for levels of C. rodentium by plating homogenates and counting the number of colonies as for Figure 6 (A/B). The tissues were also processed for immuno-fluorescence in the same manner (C/D). It can be seen that although there are still equivalent amounts of bacterial bainding, bacteria are still unable to migrate downwards in between the crypts, in the ILK-ko mice at this second time-point.