Figure 6

Effects observed in ILK-ko mice are not due to impaired binding of C rodentium . ILK-ko and control mice were gavaged with C. rodentium and their cecal and colonic tissues assessed for levels of C. rodentium by plating homogenates and counting the number of colonies, in both the colon (A) and cecum (B). The tissues were also processed for immuno-fluorescence and probed with antibodies to LPS (green) and Tir (red), and overlayed (yellow). As can be seen bacteria are capable of binding in the ILK-ko mice but may be impaired in their ability to migrate downwards in between the crypts (C/D).