Figure 3

ILK-ko mice exhibit reduced expression of the chemokine CCL2, and reduced monocyte-macrophage influx. A. CCL2 induction (RT-PCR, message compared to that of β-actin) was significantly (**p < 0.01) reduced in the ILK-ko group. B. Distal colonic lysates (pooled) were used to determine CCL2 levels by ELISA. The data are for 6 mice per group and show a reduction in levels of CCL2 in the ILK-ko mice (*p < 0.01). C. IHC for F4/80 which is a marker for cells of the monocyte/macrophage lineage shows reduced numbers in the ILK-ko mice. D. Histology of a representative section from each of the WT and ILK-ko mice shows less inflammation and hyperplasia in the ILK-ko example shown. Notably, there are elongated crypts seen in the ILK-ko mice as can be seen to the right side of the ILK-ko panel.