ILK is induced in response to C. rodentium exposure of CMT 93 cells, together with activation of Akt, and ILK regulates Akt activation in response to LPS. A. Colonic CMT 93 cells were exposed to C. rodentium for the times indicated. Cellular protein was obtained by lysing cells in homogenization buffer, and western blotting performed for the molecules indicated. B. After knocking down ILK using si-RNA (or scrambled control oligo) HCT116 cells were stimulated with LPS (1 ug/ml) for 30 min and western immunoblotting performed. GAPDH and β-actin were used as the loading controls.