Figure 8

The composition of commensal bacteria is altered in naive GC-C ^−/− mice relative to GC-C ^+/+ . Real time PCR of bacterial genomic DNA extracted from stool was performed using family-specific bacterial 16S rRNA gene primers. There was significant enrichment of Enterobacteriaceae and Atopobium in GC-C^−/− compared to GC-C^+/+ while the level of Lactobacillus was decreased. Line represents median value. (Entero., Enterobacteriaceae; Lacto., Lactobacillus; Bifido., Bifidobacteria; E. rectale, Eubacterium rectale; C. cocoides, Clostridiae cocoides; WT, wildtype GC-C^+/+; KO, GC-C^−/−; *P < 0.03, ** P < 0.005, n = 10–12 per group).